Cryopreservation of human spermatozoa with penetrating and non-penetrating cryoprotectants
نویسندگان
چکیده
منابع مشابه
Exploring the Possibility of Cryopreservation of Feline and Canine Erythrocytes by Rapid Freezing with Penetrating and Non-Penetrating Cryoprotectants
Efficient application of veterinary blood transfusion approaches for small companion animals requires readily available supply of the donor material. This can be achieved by developing of effective biobanking technologies allowing long-term storage of donor blood components via cryopreservation. Transfusion of an erythrocyte concentrate allows the successful correction of various hematological ...
متن کاملComparison of cryoprotectants and methods of cryopreservation of fowl spermatozoa.
The deleterious effects of three cryoprotectants, glycerol, dimethylsulfoxide (DMSO), and dimethylacetamide (DMA), were compared on fowl spermatozoa. The viability and integrity of spermatozoa were measured with eosin-nigrosin smears. Glycerol was the least deleterious cryoprotectant, followed by DMA, and DMSO was the most toxic. Methods employing either glycerol or DMA were then compared for t...
متن کاملComparison and Avoidance of Toxicity of Penetrating Cryoprotectants
The objective of this study was to elucidate the toxicity of widely used penetrating cryoprotective agents (CPAs) to mammalian oocytes. To this end, mouse metaphase II (M II) oocytes were exposed to 1.5 M solutions of dimethylsulfoxide (DMSO), ethylene glycol (EG), or propanediol (PROH) prepared in phosphate buffered saline (PBS) containing 10% fetal bovine serum. To address the time- and tempe...
متن کاملryopreservation of human spermatozoa : omparison of two cryopreservation ethods and three cryoprotectants iran
0 d 0 eceived November 19, 003; revised and ccepted February 17, 004. eprint requests: Ashok garwal, Ph.D., H.C.L.D., enter for Advanced esearch in Human eproduction, Infertility, nd Sexual Function, lickman Urological nstitute and Department of bstetrics-Gynecology, he Cleveland Clinic oundation, 9500 Euclid venue, Desk A19.1, leveland, Ohio 44195 FAX: 216-445-6049; Eail: [email protected]). Cen...
متن کاملNon-penetrating Sham Acupuncture
Most randomised-controlled trials of acupuncture use penetrating sham acupuncture as control intervention. Sham acupuncture is described as acupuncture at points which are not known as acupuncture points. Because of physiological changes due to skin penetration sham acupuncture should not be defined as placebo control. In 1998 a new placebo needle was introduced by Streitberger. This placebo ne...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Медицина сьогодні і завтра
سال: 2019
ISSN: 2414-4495,2710-1444
DOI: 10.35339/msz.2019.85.04.04